Affinity Chromatography of Acetylcholinesterase. The Importance of Hydrophobic Interactions
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چکیده
منابع مشابه
Hydrophobic Affinity Chromatography of Proteins
1. Chromatographic studies have been made of the affinities of a number of purified proteins for agarose (Sepharose 4B) substituted with 4-phenylbutylamine (PBA) or with E-aminocaproyl-n-tryptophan methyl ester (ACTME) as compared to controls of untreated agarose and/or of cyanogen bromide treated agarose without addition of a substituting amine. 2. At pH 8, cu-chymotrypsin (3.4.4.5) and 7s y-g...
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INTRODUCTION The Serum and Glucocorticoid regulated kinases (SGK) are a family of serine/threonine protein kinases that show extensive sequence homology to the Protein Kinase B (PKB/Akt) enzymes. Three isoforms exist in humans: SGK1, SGK2 and SGK3. Like PKB, SGK1 acts downstream of phosphatidylinositol-3-kinase (PI3K) (1). PKB requires phosphorylation of its activation loop and of a hydrophobic...
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Background: Plasminogen is one of the compounds derived from human plasma. Activation of plasminogen produces plasmin. Plasmin is able to lyse fibrinogen, fibrin, and some other human plasma proteins. The aim of the present work was to study the separation of human plasminogen by affinity chromatography using gel lysine Sepharose. Materials and Methods: Normal human plasma was used as the...
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5' tritylated oligonucleotides binding hydrophobically to low trityl cellulose/sepharose (< 15 microMTr/ml) retain their hydrogen-bonding specificities for complementary sequences. This, constitutes a novel mode of attaching affinity ligands to solid supports, is more convenient than existing methods, and proceeds with 100% yield. The salt, dielectric constant and temperature dependence of thes...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1976
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1976.tb10841.x